Mouse anti CD3 Antibody MBS570349 from MyBioSource.com

Description

UCHT1 is directed against human CD3 - the multichain complex associated with the T-cell receptor. Precursor T-cells are surface CD3 negative but positive for cytoplasmic CD3. All mature T-cells are both cytoplasmic and surface CD3 positive.

The UCHT1 antibody permits the identification and enumeration of normal and leukemic human blood and bone marrow cells using flow cytometry.

Results must be put within the context of other tests as well as the clinical history of the patient by a certified professional before final interpretation. Analysis performed with this antibody should be paralleled by positive and negative controls

Product Specs
ItemMouse anti CD3 Antibody
CompanyMyBioSource.com
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberMBS570349
Quantity0.2 mg
ApplicationsFlow Cytometry (FC/FACS), Indirect Immunofluorescence (IF)
Conjugate/TagUnconjugated
FormatPurified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3
ReactivityHuman
TargetCD3
IsotypeIgG1
Molecular Weight18,897 Da
CloneUCHT1
HostMouse
ImmunogenThe CD3 mAb (clone UCHT1) recognizes cytoplasmic CD3 epsilon in precursor T-cells and cytoplasmic and surface CD3 epsilon in mature T-lymphocytes.

The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).The sensitivity of UCHT1 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity).In practice, 50 ul of leukocytes containing 107 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity
NCBI Full Gene NameCD3g molecule S homeolog
NCBI Gene AliasesCD3, cd3-a, cd3-gamma, cd3g, cd3g-a, cd3g/d, cd3gd, t3g
ClonalityMonoclonal